補(bǔ)充谷胱甘肽可防止在碳酸氫鹽含量不同的巖砂中生長(zhǎng)的黃ca中的鐵缺乏
Zahra Gheshlaghi, Reza Khorassani, Javier Abadía, Ana Alvarez-Fernández, Adrián Luis-Villarroya, Amir Fotovat & Mohammad Kafi
Abstract
Background and aims
The effects of root glutathione (GSH) supplementation on leaf chlorophyll, Fe concentrations and contents in leaves, stems and roots, and traits associated to Fe deficiency were studied in Medicago scutellata plants grown in rock sand under conditions of Fe deficiency, in the presence of different concentrations of bicarbonate.
索引
背景和目標(biāo)
研究了補(bǔ)充谷胱甘肽（GSH）對(duì)在缺鐵條件下在缺鐵條件下在巖砂中生長(zhǎng)的黃Medi藥草中葉黃素，葉，莖和根中鐵的濃度和含量以及與鐵缺乏相關(guān)性狀的影響。不同濃度的碳酸氫鹽。
Methods
Plants were grown in acid-washed rock sand irrigated with a zero Fe solution (pH 7.8 with 0.5 g L?1 CaCO3) or a 45 μM Fe(III)-EDDHA solution (5 mM MES, pH 5.5), with 0, 5 or 15 mM NaHCO3, and 250 mL of 1 mM GSH was added daily to half of the pots.
方法
植物在酸洗過的巖砂中生長(zhǎng)，用零鐵溶液（pH 7.8和0.5 g L -1 CaCO 3）或45μMFe（III）-EDDHA溶液（5 mM MES，pH 5.5）灌溉，每天將5或15 mM NaHCO 3和250 mL 1 mM GSH添加到一半的鍋中。
Results
Iron deficiency caused characteristic symptoms in plants, with GSH supplementation relieving them. Glutathione supplementation led to increases in total Fe, chlorophyll and leaf total and extractable Fe, whereas root Fe concentrations decreased. Traits associated to Fe deficiency, including changes in biomass, root morphology, carboxylate contents and antioxidant parameters became less intense with GSH supplementation.
結(jié)果
缺鐵會(huì)引起植物特征性癥狀，補(bǔ)充GSH可以緩解這些癥狀。補(bǔ)充谷胱甘肽導(dǎo)致總鐵，葉綠素和葉片總鐵和可提取鐵的增加，而根鐵的濃度下降。補(bǔ)充谷胱甘肽與鐵缺乏相關(guān)的性狀，包括生物量，根系形態(tài)，羧酸鹽含量和抗氧化劑參數(shù)的變化，變得不那么強(qiáng)烈。
Conclusions
Glutathione supplementation allowed plants to take up Fe from the rock sand via a reductive solubilization mechanism. Also, the distribution of Fe within the plant changed, with more Fe being allocated to the shoot tissues and less to the roots.
結(jié)論
谷胱甘肽的補(bǔ)充使植物通過還原增溶機(jī)理從巖砂中吸收鐵。同樣，植物中鐵的分布發(fā)生了變化，更多的鐵分配給了芽組織，而更少的鐵分配給了根。
